Establishment of caco-2 cell model
WebThe capacity to secrete mucus is fundamental to the establishment of a physiologically relevant in vitro intestinal model. ... Raub TJ, Borchardt RT. Characterization of the human colon carcinoma cell line (Caco-2) as a model system for intestinal epithelial permeability. ... Alpers DH. Caco-2 cells express a combination of colonocyte and ... WebApr 1, 2013 · In vitro cell culture models for studying oral drug absorption during early stages of drug development have become a useful tool in drug discovery and development, with respect to substance throughput and reproducibility. The aim of this study was to establish an in vitro cellular model based on human colon carcinoma Caco-2, mucus …
Establishment of caco-2 cell model
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WebResults: When exposed to 1, 2 and 3 mmol/L of t-BHP for 3 to 6 hours, the oxidative stress model of Caco-2 cells from the F15 generation could be established steadily. The … WebAug 25, 2024 · The Caco-2 cell model has been widely used to assess the permeability of drug candidates. It has provided a high throughput in vitro platform, functionally resembling the enterocytes. Since the oral route is the most preferred for drug administration, the Caco-2 cell model acts as a very important t …
WebMar 30, 2024 · The Caco-2 model is a common cell model for material intestinal absorption in vitro, which usually takes 21 days to establish. Although some studies have shown that adding puromycin (PM) can shorten the model establishment period to 7 days, this still requires a long modeling time. Therefore, explor … WebThe tight junction formation in Caco-2 cell monolayers was compared after 9 and 13 d of culture. Four different sized paracellular markers were simultaneously applied to the apical side of the monolayers. ... Evaluation of the establishment of a tight junction in Caco-2 cell monolayers using a pore permeation model involving two different sizes ...
WebDec 1, 2007 · Caco-2 cell monolayer model was established and evaluated by morphology feature using scanning electron microscope, inverted microscope and transepithelial … WebOct 1, 2004 · The Caco-2 cell model responded to TA addition of up to 100 mg/meal in a pattern similar to that in humans (Table 2; Fig. 2). The logarithms of the absorption ratios (meals with TA/meals without TA) in Caco-2 cells and in human studies were correlated ( R = 0.927; P = 0.007).
WebMar 30, 2024 · The Caco-2 model is a common cell model for material intestinal absorption in vitro, which usually takes 21 days to establish. Although some studies have shown that adding puromycin (PM) can ...
WebRh123, a known P-gp substrate, was added to the Caco-2 cells to evaluate the impact of any potential interactions of G-Rb3 and FGL on pharmacokinetics. Verapamil is one of the widely recognized P-gp inhibitors and it was applied as a control in this study . ... After the establishment of the MI model, ... leather peep toe shoesWebDec 1, 2009 · To better simulate the major presence of columnar absorptive cells relative to the goblet cells in the intestinal epithelium, cocultures of the two human intestinal cell lines (Caco‐2 and HT29‐MTX) have been tested to produce a more physiological model, mimicking the major cell phenotypes present in the intestinal epithelium (Pontier et al ... leather pen case memoWebJun 18, 2012 · Cell-based in vitro drug permeation models are very attractive because they are amenable to high-throughput screening and can be implemented at a relatively low cost compared to the in vivo models [].Caco-2 in vitro cell culture model is a well-established and widely used model to characterize the human intestinal permeability of compounds. … leather peeling from couchWebApr 13, 2024 · In comparison to acarbose, compounds 1 and 2 inhibited α-glucosidase activity more effectively in Caco-2 cells without cytotoxicity at a concentration of 62.5 µg/mL. Furthermore, the glucose uptake pathways mediated by SGLT1, GLUT2, and GLUT5- were downregulated in Caco-2 cells treated with compounds 1 and 2. leather pencil cupWebAug 31, 2024 · The Caco-2 monolayer is the most widely used in vitro model of the human intestinal mucosa to study absorption. However, models lack communication from other cells present in the native intestine, such as signals from fibroblasts in the lamina propria. In this study, we have investigated the effects of fibroblasts upon the Caco-2 epithelium … leather peep toe shoes for womenWebAug 25, 2024 · The Caco-2 cell model has been widely used to assess the permeability of drug candidates. It has provided a high throughput in vitro platform, functionally … leather pencil holderWebCaco-2 cells are a cell model of small intestinal enterocytes (Sambuy et al., 2005 ). Caco-2 cells allow independent examination of the effects of calcium on iron absorption of the … leather peep toe flats